cell culture murine fl83b hepatocytes  (ATCC)

Journal: ACS biomaterials science & engineering

Article Title: Carcinomembrane-Camouflaged Perfluorochemical Dual-Layer Nanopolymersomes Bearing Indocyanine Green and Camptothecin Effectuate Targeting Photochemotherapy of Cancer.

doi: 10.1021/acsbiomaterials.4c01150

Figure Lengend Snippet: Figure 2. Characterization of the MICFNS. (A, B) SEM images of ICFNS (A) and MICFNS (B) at magnifications of 10,000× and 60,000× (inset). The inset photographs showing green, emulsified solutions exhibit the real appearance of ICFNS (A) and MICFNS (B) samples. (C, D) TEM images of ICFNS (C) and MICFNS (D). (E, F) Representative size distribution (E) and zeta potential (F) of the extruded BCM, ICFNS, and MICFNS measured by DLS. (G) Encapsulation and loading ratios of ICG and CPT in MICFNS. Values are the mean ± SD (n = 3). (H, I) Variation of size (H) and density (I) of MICFNS under incubation at 4 or 37 °C for 7 days. Values are the mean ± SD (n = 3). (J) Western blot images of N-cadherin, CD47, and GAPDH proteins expressed from the B16F10 cell lysis, BCM, and MICFNS. (K) Hemocompatibility of ICFNS and MICFNS with different doses to murine erythrocytes. Values are the mean ± SD (n = 3). (L) Verification of the homology of MICFNS. The intensities of the ICG-derived fluorescence expressed from the FL83B and B16F10 cells were detected by spectrofluorometry performed with 750/ 838 nm excitation/emission wavelengths and were quantitatively represented by RFUs. Values are the mean ± SD (n = 3). *P < 0.05.

Article Snippet: Murine hepatocyte FL83B cells (ATCC) were cultured using 90% Ham’s F12K medium supplemented with 10% FBS, 1.5 g/L sodium bicarbonate, 2 mM L-glutamine, and 100 U/mL penicillin/streptomycin at 37 °C with 5% CO2.

Techniques: Zeta Potential Analyzer, Encapsulation, Incubation, Western Blot, Lysis, Derivative Assay, Fluorescence